Table 1 Seven P450 enzymes of the glucosinolate pathway from four different plants were targeted for engineering in our study. CYP79s control the amino acid substrate entering the pathway and we selected five with distinct substrate preferences and plant origin. The subsequent CYP83s are promiscuous and convert any oximes generated by CYP79s. ΔTM and ΔTM + represent the residues that were truncated after the membrane anchor or after the membrane anchor plus the hydrophilic region. Complete amino acid sequences of the enzymes can be found in Table S5

From: Systematic engineering pinpoints a versatile strategy for the expression of functional cytochrome P450 enzymes in Escherichia coli cell factories