Table 3 Strains and plasmids used in this work
Strains | Features | References |
|---|---|---|
Streptomyces rimosus | ||
 ATCC 10970 | WT (R7) | [9] |
 ATCC 10970 bhi | WT with bhimamycin cluster | This work |
 HP 126 | Oxytetracycline hyperproducing mutant (HP) | [18] |
 HP 126 bhi | HP 126 with bhimamycin cluster | This work |
 HP 126 ∆v3 | HP 126 with deleted oxytetracycline cluster (DV3) | This work |
 HP 126 ∆v3 bhi | HP 126 ∆v3 with bhimamycin cluster | This work |
Escherichia coli | Â | Â |
 GB05-red | Strain used for Red/ET cloning | [89] |
 GB05 | General cloning strain | [89] |
 ET12567 pUB307 | Donor strain for intergeneric conjugation | [90] |
Plasmids | ||
 R3A4 | Cosmid containing S. rimosus chromosome fragment with otc cluster | This work |
 pHSU-OTC3 | Derivative of R3A4 with otc cluster replaced by hyg and ery resistance cassettes | This work |
 patt-sHyg-ery-oriT | Resistance cassette plasmid containing a synthetic fragment with hyg, ery, oriT, B-CC, P-GG, and loxP sites | This work and [65] |
 pUWLint31 | Plasmid containing phiC31 integrase | [65] |
 1-C9_int_act | Fosmid derivative with a bhimamycin A cluster containing fragment from the chromosome of Frankia sp. CcI3 | [28] |