Fig. 8
Multiomics profiling of oxytetracycline-producing S. rimosus. The data show log2-fold changes in the expression of genes encoding enzymes of major catabolic and anabolic pathways and the biosynthesis of oxytetracycline and other malonyl-CoA-derived secondary metabolites in the mutant HP126 (HP) during the production phase (24 h). The expression levels of wild-type S. rimosus ATCC 10970 (R7) were used as a reference. Regarding the metabolic pathway repertoire, genome sequencing revealed the deletion and multiplication of different genes. Absent genes in the mutant are marked by a red cross, while a thicker frame around the corresponding rectangle represents an increase in the copy number. The exact copy number for each gene is summarized in Additional file 2. Bubble charts represent the absolute intracellular concentrations of CoA-thioesters and their changes compared to that of S. rimosus ATCC 10970. The displayed enzymes were assigned to specific pathways based on KEGG pathway maps and the annotations obtained during DNA and RNA sequencing. n = 3