Fig. 7

Metabolic engineering of S. rimosus for heterologous production of the polyketide bhimamycin. LC‒MS analysis of crude extract from S. rimosus HP126 Δv3 and S. rimosus HP126, showing the extracted ion chromatogram for 461.15–461.20 Da (RT 2.62), which reveals the absence of oxytetracycline in the deletion strain (A). Chemical structure of bhimamycin (B). Final levels of the product from cultures of S. rimosus ATCC 10970, S. rimosus HP126, and S. rimosus Δv3  (C).