Fig. 6

Integrated analysis of oxytetracycline production in S. rimosus at the transcriptome and metabolome levels. The studied strains comprise the wild type S. rimosus ATCC 10970 (R7), its mutagenized oxytetracycline-hyperproducing derivative HP126 (HP), and its non-producing counterpart HP126 ∆v3 (DV3). Differences in the expression of genes associated with the biosynthesis and withdrawal of acetyl-CoA and malonyl-CoA during the major production phase (A). The differences are represented as M-values between strain HP versus R7 (y-axis) and strain DV3 versus R7 (x-axis) so that correlations between all strains can be inferred from the plot. Absolute intracellular levels of CoA thioesters (B, C). The metabolites were analysed in all strains at the following time points: 8 h (T1), 16 h (T2), 36 h (T3), and 50 h (T4), while the final sample (T5) was taken after 60 h (R7), 55 h (HP), and 72 h (DV3). The statistical significance of the observed differences in concentrations was estimated by a t-test: NS, not significant; p < 0.05, *; < 0.01, **). n = 3