Fig. 3

Genomic profiling of the wild type S. rimosus ATCC 10970 (R7), its mutagenized oxytetracycline-hyperproducing derivative HP126 (HP), and the chassis strain HP126 ∆v3 (DV3), created by deletion of the oxytetracycline cluster. The data show the genomic repertoire of the linear chromosome and the plasmid of the three strains (A) and the distribution of gene expression across the different genomic regions (B). The different chromosomal and plasmid-encoded segments are numbered from 1 to 5. These segments are shown as arrows to visualize their position and orientation in the wild type and their rearranged position and orientation in the mutants, as determined by BLASTN comparison. Deleted genomic regions that were lost during the mutagenesis process are shown in yellow. The oxytetracycline cluster is shown in light blue. Single nucleotide polymorphisms (SNPs) between the strains are indicated by colour. The gene expression data reflect the average expression of all genes that belong to a specific genomic region. The M-value reflects the difference in expression in strains HP and DV3 compared to the wild type. Green and red arrows highlight expression changes superimposed by genomic rearrangement events, such as multiplication and deletion. LTR, long terminal repeat; D, deletion; IR, inverted repeat; ME, mobile element