Table 2 Strains used in this study

Strains

E. coli DH5α

F−, endA1, hsdR17 (rk-mk-), supE44, thi1, recA1,

gyrA, (Nalr), relA1, D (lacZYAargF), U169,

and F 80lacZDM15

Lab stock

B. subtilis 168

Lab stock

GBACB

B. subtilis 168; ΔamyE::P_grac-SATp-P₄₃-SMT; ΔxylA::P_grac-SAM2-SMT

[10]

GBACB-pSTOP

B. subtilis GBACB; pSTOP1622

This work

GBACB-NmMmt

B. subtilis GBACB; pSTOP1622- NmMmt

This work

GBACB-RiMmt

B. subtilis GBACB; pSTOP1622- RiMmt

This work

GBACB-CpMmt

B. subtilis GBACB; pSTOP1622- CpMmt

This work

GBACBM

B. subtilis GBACB; ΔKinB::P_xylA-NmMmt

This work

HT

B. subtilis 168; pHT01

[10]

HTSATp

B. subtilis 168; pHT-SATp

[10]

GMC1

B. subtilis GBACB; ΔKinB::P_xylA-NmMmt ; ΔiscSB

This work

GMC2

B. subtilis GBACB; ΔKinB::P_xylA-NmMmt ; ΔaspB

This work

GMC3

B. subtilis GBACB; ΔKinB::P_xylA-NmMmt ; ΔyhdR

This work

GMS

B. subtilis GBACB; ΔKinB::P_xylA-NmMmt ; ΔsdaA

This work

GMSC

B. subtilis GBACB; ΔKinB::P_xylA-NmMmt ; ΔiscSB ; ΔsdaA

This work

Plasmids

pSTOP1622

Amp^r, Tet^r, E. coli-B. subtilis shuttle vector

[49]

pSTOP1622- NmMmtN

pSTOP1622 derivate with NmMmt cloned

This work

pSTOP1622- RiMmtN

pSTOP1622 derivate with RiMmt cloned

This work

pSTOP1622- CpMmtN

pSTOP1622 derivate with CpMmt cloned

This work

pHT-XCR6

pHT01 derivate, XylR-P_xylA-FnCpf1-NgAgo (D663A, D738A, truncated to 650–887 aa)

[44]

pcrF11

ColE1 Kan^r, RepF Kan^r, E. coli-B. subtilis shuttle vector, crRNA insertion under P_veg

[44]