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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Optimized methyl donor and reduced precursor degradation pathway for seleno-methylselenocysteine production in Bacillus subtilis

Fig. 2

Screening exogenous Mmts for provision of MMet as a methyl donor for SeMCys production. A, Effect of expressing Mmts on SeMCys-specific production. All genes were codon-optimized based on B. subtilis and chemically synthesized. RiMmt was obtained from Roseovarius indicus, NmMmt from Novosphingobium sp. MBES04, and CpMmt from Candidatus Peregrinibacteria. B, SDS-PAGE analysis of the heterologous Mmts overexpressed in B. subtilis. White arrow shows the bends of target proteins. C, Influence of genome-integrated NmMMT gene on SeMCys production

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Microbial Cell Factories

ISSN: 1475-2859

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