From: Engineering Comamonas testosteroni for the production of 2-pyrone-4,6-dicarboxylic acid as a promising building block
Component
Concentration/Value
Carbon source:
- TPA
- Glycolic acid
10 mM
25 mM
(NH4)2SO4
Adjusted to C:N ratio of 10
MgCl2.7H2O
0.5 g/L
Phosphate buffer
75 mM
pH
7