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Table 3 Primer sequences used in this study

From: A strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases

Name

Forward primer (5′ → 3′)

Name

Reverse primer (5′ → 3′)

pE-5-f

GCGAACCATATGGCAGCCAACAATG

pE-5-r

AGCCGCGTCGACGGTGATAGAAAGAGTC

pE-6-f

GAGTGACATATGAAAACTATTGAAG

pE-6-r

TCCATGGTCGACGAGTTGGTAGTTGGAACTGA

pE-9-f

GCACGTCATATGTTTCTCACACTCT

pE-9-r

CACGCTGTCGACCCGCACTGAGGAGTTGATTA

pX-5-f

AAACAGAAGCTTAGAAAGAGGCTAG

pX-5-r

AGGCCAGAATTCCCTGAGGTGATAGAAAGAGT

pX-6-f

TCACCGCTGCAGATAAATTAATTGG

pX-6-r

CAGTATGAATTCAGGTCAAATGATGAAACTTA

pX-9-f

AGTACTTCTAGATGCAAATCTAGTA

pX-9-r

CAACCGCCCGGGGGAGTTGATTAACCTGCACG

pK-5-uf

CCAGCTGAATTCGCGCAATCTACGGTGGCA

pK-5-ur

AAGTAGACTAGTAGACTCTTTCTATCACCT

pK-5-df

ATGGGGACTAGTTAGCCTCTTTCTTTGTAG

pK-5-dr

GATTTTTCTAGAAGGGCTTTTTATCAGGAC

pK-6-uf

AACGCCGAATTCGCCGTATCCCTCGTA

pK-6-ur

TCACGCTCTAGAGTTCCTGCGTCCCT

pK-6-df

GGCAGCTCTAGAGCCACCGAAAGTCC

pK-6-dr

TTGAAACTGCAGAAGACCCTTCCAGATT

pK-9-uf

CAACGTGGATCCAAATGCTGTGCTGGAATA

pK-9-ur

AAGTACTCTAGAGCGTGGGCTGATTGTA

pK-9-df

CCTCCATCTAGAATTCTCCTGCGTCGTCT

pK-9-dr

CCACCTAAGCTTACGCCATTCTTCTCCC

lac-X-f

CCGTCTTCTAGATGAAAAGAAAAACCAC

lac-X-r

GCGAGATCTAGAAAATAGCGGCAAAAATAA

lac-S-f

CCGTCTACTAGTTGAAAAGAAAAACCAC

lac-S-r

GCGAGAACTAGTAAATAGCGGCAAAAATAA

  1. Primer lac-X series amplified lacZ in pK18_la_tesB and pK18_la_te9. Primer lac-S series amplified lacZ in pK18_la_tesA. Genomic PCR verification of the knockouts was performed using the primer pK -uf and pK -dr series