A strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases

Table 2 Plasmids used in this study

Plasmid	Relevant genotype	Source
pK18mobsacB	Kan^R, sacB; suicide plasmid for gene knockout in C. glutamicum	Lab collection
pET-28a	Kan^R; plasmid for heterologous protein expression in E. coli, control-lable T7 promoter	Lab collection
pXMJ19	Cm^R; E. coli–C. glutamicum shuttle plasmid for complementary overexpression in C. glutamicum, Tac promoter	Miaoling Bio
pET_tesA	Kan^R; pET-28a carrying tesA	This study
pET_tesB	Kan^R; pET-28a carrying tesB	This study
pET_te9	Kan^R; pET-28a carrying te9	This study
pK18_tesA	Kan^R; pK18mobsacB carrying the upper and lower homologous arms of tesA	This study
pK18_tesB	Kan^R; pK18mobsacB carrying the upper and lower homologous arms of tesB	This study
pK18_te9	Kan^R; pK18mobsacB carrying the upper and lower homologous arms of te9	This study
pK18_la_tesA	Kan^R; pK18_tesA carrying lacZ (NC_000913)	This study
pK18_la_tesB	Kan^R; pK18_tesB carrying lacZ (NC_000913)	This study
pK18_la_te9	Kan^R; pK18_te9 carrying lacZ (NC_000913)	This study
pX_tesA	Cm^R; pXMJ19 carrying tesA	This study
pX_tesB	Cm^R; pXMJ19 carrying tesB	This study
pX_te9	Cm^R; pXMJ19 carrying te9	This study

The recombinant plasmids were used as follows: The pet_ series was used for thioesterase heterologous expression in E. coli BL21(DE3). The pK18_la_ series was used for gene knockout in C. glutamicum. The pX_ series was used for complementary overexpression in the knockout

ISSN: 1475-2859