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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis

Fig. 1

Scheme for enzymatic detection of creatinine. Creatinine is converted by three enzymes in sequential cascade: creatininase, creatinase, and sarcosine oxidase. First, creatininase catalyzes creatinine to creatine; creatinase catalyzes hydrolysis of creatine in the second step; sarcosine oxidase catalyzes sarcosine to detectable hydrogen peroxide (H2O2) in the third step. Horseradish peroxidase catalyzes H2O2 to generate a purple color caused by a coupler reagent 4-AA (4-Aminoantipyrine) and a color-generating substance TOOS (N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline)

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Microbial Cell Factories

ISSN: 1475-2859

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