Fig. 6From: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case studyEffect of different signal peptide on the lipase activity of periplasmic protein preparation. The different strains (from BL21-01 to BL21-06) were grown at 37 °C until the value of OD600 reached 0.8, and then induced with 0.2 mM IPTG for 40 h at 20 °CBack to article page