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Table 2 Transcription profiles of CYPs under the soybean oil and the control conditions

From: Integrating multi-omics analyses of Nonomuraea dietziae to reveal the role of soybean oil in [(4′-OH)MeLeu]4-CsA overproduction

Gene name

∆CtC1

−∆∆Ct

C1

C2

S1

S2

S1 vs. C1

S2 vs. C2

CYP-sb1

19.08

16.06

ND

18.64

–

−2.58

CYP-sb10

ND

17.28

ND

ND

–

–

CYP-sb11

13.01

12.45

13.00

13.36

0.01

−0.91

CYP-sb12

6.00

9.23

3.32

8.82

2.68

0.41

CYP-sb13

20.63

20.80

18.18

11.33

2.45

9.47

CYP-sb15

7.05

5.38

6.39

8.24

0.66

−2.86

CYP-sb16

ND

20.66

ND

ND

–

–

CYP-sb17

ND

17.08

14.53

10.85

–

6.23

CYP-sb2

9.16

10.90

9.79

12.55

−0.63

−1.65

CYP-sb20

9.13

10.70

9.54

8.26

−0.41

2.44

CYP-sb21

8.22

11.11

4.28

4.96

3.94

6.15

CYP-sb22

9.78

10.04

7.20

10.83

2.58

−0.79

CYP-sb23

ND

12.28

13.87

12.59

–

−0.31

CYP-sb24

ND

ND

11.94

12.56

–

–

CYP-sb3-1

ND

15.17

ND

ND

–

–

CYP-sb3-2

16.76

11.63

12.07

8.40

4.69

3.23

CYP-sb4

ND

22.32

ND

11.90

–

10.42

CYP-sb6

19.99

ND

14.38

17.59

5.61

–

CYP-sb7

ND

12.85

17.41

ND

–

–

CYP-sb8

12.15

11.64

8.32

12.31

3.83

−0.67

CYP-sb9

9.35

8.91

7.89

10.13

1.46

−1.22

  1. C1, C2, S1 and S2 stand for the samples of 36 and 48 h under the control condition and the soybean oil condition, respectively
  2. ∆Ct: The difference value of Cycle Time between the targeted gene and the reference gene. ∆Ct = Ct(Targeted gene) − Ct(16S rDNA). A lower ∆Ct represents a higher relative transcription level. −∆∆Ct = log2(fold change)
  3. Each data was calculated the mean value of six samples (three biological repeations and two technical repeations)
  4. ND not detected
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Microbial Cell Factories

ISSN: 1475-2859

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