Fig. 3From: Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturasesDetermination of expression levels of D12, D61 and D62 genes by RT-qPCR in the overexpressing strains (Mc-D12, Mc-D61 and Mc-D62), and the control strain Mc-1552. Strains were grown in a 2 l fermenter with 1.5 l K&R medium, and the mycelium was harvested at 24, 48, and 72 h. Total RNA of strains at different time was extracted and verified by RT-qPCR. Values were mean of three independent fermentation experiments. Error bars represent the standard error of the meanBack to article page