Table 2 Primer list used in this study
Oligonucleotide | Primer sequence (5′–3′) | Purpose |
|---|---|---|
Homo arm-aprR cassette | ||
F | TACCCGGCGGAGGCGTACCACCAGCAGTATCTGGACAAGAAATTCCGGGGATCCGTCGACC | Construction of pMSCPik311 |
R | AGCGGCGCAGCCTCATGTAACGGGCCTGAGAGTGGCAGAAGCTTGCATTGTAGGCTGGAGCTGCCTC | |
AprR cassette check | ||
F | TACGGCGAGATCACCACGAC | Check for insertion of apr R cassette |
R | GCGAGGATCGAGACGCGTGA | |
PikD-kan | ||
F | ACAACACCAAGCTTGCCCGCCT | Construction of pSAPDK |
R | ACTGCAAGCTAGGCCCACCGATGAAGGACA | |
F | TCGGTGGGCCTTAGCTTGCAGTGGGCTTACA | |
R | TTGGTCGGTGAATTCGAACCCCAGA | |
PikD-kan check | ||
F | AGGCCCTGTTCGACGACCGA | Confirmation of pPik001 |
R | TGCCTCGTCCTGCAGTTCATTCA | |
PikRI-AI | ||
F | CCCCTTCGAATTTCCGCCGC | Confirmation of pPik001 |
R | CCGCCAGGAGTTCCCAGAAC | |
PikAII | ||
F | ACCGGTCTCAACTTCCGCGA | Confirmation of pPik001 |
R | TCCAGGAGGGACAGGGTCAGC | |
PikD | ||
F | TCGCGGCACGGCATGGAGTACATGCA | Confirmation of pPik001 |
R | TCAGGCCGTGACGGACTTGTCCT | |
PikAI RNA | ||
F | GACTGGGACCGCTTCTACCT | qRT-PCR |
R | TACGAGACCGAGGAGGATCT | |
DesIII RNA | ||
F | GCTCTTTCTGGACCGCATAG | qRT-PCR |
R | CGGTCATTTCGAAGCAGATT | |
DesVI RNA | ||
F | CATCTGGAGCACTTCACCAA | qRT-PCR |
R | CTCGGTCGTCTTCAGGTAGC | |
PikC RNA | ||
F | GCTATCTCTCCCGGCTCATC | qRT-PCR |
R | GATTGACCGTGGTCTCGTG | |
PikD RNA | ||
F | GGACGCTCTTACTCGTCTCC | qRT-PCR |
R | CGGAGTAACTGGCAGAGGAC | |
HrdB RNA | ||
F | GCGGTGGAGAAGTTCGACTA | qRT-PCR |
R | CACATGGTCGAGGTCATCAA | |