Table 1 Bacterial strains and plasmids used in this study
Strain/plasmid | Relevant characteristics | Source/references |
|---|---|---|
Plasmid | ||
pSBAC | aacIII(IV), oriT, attP-int ΦBT1, backbone of pCC1BAC | [11] |
pSAPDK | Modified pSBAC with deleted attP-int ΦBT1 and inserted Kan R and pikD fragment | This work |
pPik | pSAPDK with 60 kb DNA insert containing whole pik gene cluster | This work |
pPik001 | pPik with attP-int ΦBT1 | This work |
pPik003 | pPik101, which replaced Hyg R with Apr R | This work |
E. coli | ||
EPI300 | F-mcrA-D (mrr-hsdRMS-mcrBC) trfA host for cloning and amplification of various BAC vectors and constructs derived from it | Epicenter |
S17-1 | E. coli host for transferring various plasmids into Streptomyces via conjugation | |
ET12567/pUZ8002 | E. coli host for transferring various plasmids into Streptomyces via conjugation | |
Streptomyces venezuelae | ||
ATCC 15439 | Original pikromycin-producing strain | [15] |
15439-HindIIIBAC | ATCC 15439 with pSAPDK and HindIII recognition sequences in a flanking region of pikromycin biosynthetic gene cluster | This work |
Streptomyces lividans | ||
TK21 | Non pikromycin-producing strain | |
Pik101 | TK21 with pPik001 | This work |
Pik102 | Pik101 with pPik003 | This work |
Streptomyces coelicolor | ||
M145 | Non pikromycin-producing strain | |
Pik201 | M145 with pPik001 | This work |