Table 1 Strains and plasmids used in this study
Name | Description | Source |
|---|---|---|
Strains | ||
 M. neoaurum ATCC 25795 | Type strain of M. neoaurum | ATCC |
  ΔmmpL3 | mmpL3 deleted in M. neoaurum ATCC 25795 | This study |
  ΔmmpL3+mmpL3 | mmpL3 complemented in strain ΔmmpL3 | This study |
  WIII | kshAs, hsd4A, kstD1, kstD2 and kstD3 deleted in M. neoaurum ATCC 25795 | [30] |
  WIIIΔmmpL3 | kshAs, hsd4A, kstD1, kstD2, kstD3 and mmpL3 deleted in M. neoaurum ATCC 25795 | This study |
  WIIIΔmmpL3-cyp125 -choM1-fadA5 | cyp125, choM1and fadA5 overexpressed in strain WIIIΔmmpL3 | This study |
Plasmids | ||
 p2NIL | Vector of two homologous arms for allelic recombination in mycobacteria, Kan R | [37] |
  p2N-mmpL3 | p2NIL carrying two homologous arms of mmpL3, Kan R | This study |
 pGOAL19 | Hyg, Pag85-lacZ, P hsp60 -sacB, PacI cassette vector, Amp R | [37] |
  p19-mmpL3 | p2NIL-derived with selection cassette from pGOAL19 for deletion of mmpL3 in mycobacteria | This study |
 pMV261 | Shuttle vector of Mycobacterium and E. coli, carrying the heat shock (hsp60) promoter, Kan R | Dr. W. R. Jacobs Jr. |
  p261-choM1 | Recombinant pMV261, for overexpression of ChoM1 in mycobacteria | This study |
  p261-choM2 | Recombinant pMV261, for overexpression of ChoM2 in mycobacteria | This study |
  p261-cyp125 | Recombinant pMV261, for overexpression of CYP125 in mycobacteria | This study |
  p261-fadA5 | Recombinant pMV261, for overexpression of FadA5 in mycobacteria | This study |
  p261-cyp125-choM1-fadA5 | Recombinant pMV261, for overexpression of CYP125, ChoM1, and FadA5 in mycobacteria | This study |
 pMV306 | Integration vector in Mycobacterium, without promoter, Kan R | Dr. W. R. Jacobs Jr. |
  p306-mmpL3 | pMV306-Phsp60-mmpL3, integrative into mycobacterial chromosomal DNA | This study |