Table 1 Strains and plasmids used in this study
Strains and plasmids | Relevant genotype and characteristics | Source |
|---|---|---|
Strains | ||
B. amyloliquefaciens NK-1 | LL3 derivative, ΔpMC1, Δupp | [12] |
B. amyloliquefaciens NK-1 (pHT315-gdh) | B. amyloliquefaciens NK-1 with the expression plasmid pHT315-gdh | This work |
B. amyloliquefaciens NK-1 (pHT315-cgdh) | B. amyloliquefaciens NK-1 with the expression plasmid pHT315-cgdh | This work |
B. amyloliquefaciens NK-1 (pHT01 + pCB-Pxyl) | NK-1 derivative with the plasmids pHT01 and pCB-Pxyl | This work |
B. amyloliquefaciens NK-1 (pHT01-xylR + pCB-Pxyl) | NK-1 derivative with the expression plasmids pHT01-xylR and pCB-Pxyl | This work |
B. amyloliquefaciens NK-TP | NK-1 derivative with its native odhA promoter replaced by the Pxyl promoter | This work |
B. amyloliquefaciens NK-TP (pHT01-xylR) | NK-TP derivative with the expression plasmid pHT01-xylR | This work |
B. amyloliquefaciens NK-PO1 | NK-1 derivative with its native odhA promoter replaced by the PO1 promoter | This work |
B. amyloliquefaciens NK-PO1 (pHT01-xylR) | NK-PO1 derivative with the expression plasmid pHT01-xylR | This work |
B. amyloliquefaciens NK-PO1 (pHT01-xylR + pHT315-gdh) | NK-PO1 derivative with the expression plasmids pHT01-xylR and pHT315-gdh | This work |
B. amyloliquefaciens NK-PO1 (pHT01-xylR + pHT315-cgdh) | NK-PO1 derivative with the expression plasmids pHT01-xylR and pHT315-cgdh | This work |
C. glutamicum ATCC13032 | Glutamic acid producing strain | Lab stock |
E. coli DH5α | F−, φ80dlacZΔM1, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(r −k , m +k ), phoA, supE44, λ− thi-1, gyrA96, relA1 | Lab stock |
E. coli GM2163 | F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (Str r ) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 mcrA | Lab stock |
Plasmids | ||
pKSU | pKSV7 derivation with upp gene expression cassette | [15] |
pHT01 | Cmr, IPTG inducible expression vector for Bacillus | MoBiTec |
pHT315 | Emr, IPTG inducible expression vector for Bacillus | [32] |
pCB | pHT315 derivation with the bgaB expression cassette | [13] |
pKSV7-Pxyl | p-KSU derivation with insertion fragment Pxyl | This work |
pHT01-xylR | pHT01 derivative with the xylR gene | This work |
pCB-Pxyl | pCB derivation with the Pxyl promoter upstream the bgaB gene | Lab stock |
pHT315-gdh | pHT315 derivation with the condon optimized gdh gene | This work |
pHT315-cgdh | pHT315 derivation with the gdh gene from C. glutamicum ATCC13032 | This work |