Fig. 1

Setup of the bioscope labeling experiment. a The bioscope has inflows from the bioreactor (flow 0.14 mL/min) and the BioScope feed (0.56 mL/min). b The BioScope feed contains labeled succinic acid matching the concentration of succinic acid of the bioreactor broth. The solution also matches the residual glucose concentration (unlabeled). This setup leads to a dilution of the biomass concentration while the residual concentration of glucose and succinic acid are similar to the BR cultivation. Using labeled succinic acid allows for immediate labeling with about 80% enrichment