Table 1 Optimum culture conditions standardised for the induction of FAD, EPOX and EH proteins
From: In vitro synthesis of 9,10-dihydroxyhexadecanoic acid using recombinant Escherichia coli
Gene | Optimum temperature (°C) | Bacterial strain | Orbital shaking (rpm) |
|---|---|---|---|
FADa | 22 | BL21(DE3)-Gold | 150 |
EPOX | 30 | BL21(DE3)CodonPlus-RIL | 150 |
EH | 30 | BL21(DE3)CodonPlus-RIL | 150 |