Fig. 3
From: In vitro synthesis of 9,10-dihydroxyhexadecanoic acid using recombinant Escherichia coli
a 12% SDS PAGE showing induction of three genes at 16, 30 and 37 °C using E. coli strain BL21(DE3)CodonPlus-RIL. Lane 1 un induced whole cell lysate; Lane 2–4 induced whole cell lysate of EPOX, EH and FAD respectively. b 12% SDS PAGE showing induction of FAD at 22 °C using E. coli strain BL21(DE3)CodonPlus-RIL, BL21(DE3)-Gold and BL21(DE3)-pLysS. Lane 1 uninduced whole cell lysate; lane 2 induced whole cell lysate. M Molecular weight marker. c 12% SDS PAGE showing the secretion of induced proteins into inclusion bodies for two proteins EPOX and EH at 30 °C. Lane 1 uninduced whole cell; Lane 2 induced cell pellet; Lane 3 induced supernatant. Arrow indicates the induced protein