Fig. 2From: Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing abilityExpression of Zhd101 in Lactobacillus reuteri cells. a RT-PCR for Zhd101 in Lb. reuteri Pg4, Lb. reuteri pNZ-zhd101, and Lb. reuteri pNZ3004, along with the housekeeping gene 16S rRNA. b Western blot for purified recombinant Zhd101 and Zhd101 in the intracellular extracts of Lb. reuteri Pg4, Lb. reuteri pNZ3004, and Lb. reuteri pNZ-zhd101. The samples (2 μg of protein in each lane) were separated by SDS-PAGE with a 12.5% gel and probed with mouse polyclonal anti-zhd101 antibody and horseradish-peroxidase-linked anti-mouse IgG antibody as the primary and secondary antibodies, respectivelyBack to article page