Fig. 5
Co-isolation of PduP-tagged protein with purified BMCs. a SDS-PAGE and anti-His6 Western blot of purified BMCs from Ec3087. As negative and positive controls, BMCs were also purified from Ec0087 (no BMCs) and Ec3090 (PduP-mCherry + full Pdu), respectively. BMCs were purified from cells at 3 h post-rhamnose induction. Induction conditions are identified at the top of the gel. 10 µg of BMCs from Ec3087 and Ec3090 and 1 µg of mock BMCs from Ec0087 were loaded on the SDS-PAGE. 1 µg of each BMC sample was analyzed by Western blot. Sizes of molecular weight standards in kDa are shown on the left. b TEM analysis of isolated BMCs from Ec3087 and Ec3090. Scale bars are 50 nm