Table 1 Primers used for the amplification and fusion of the katX2 gene with the spectinomycin resistance gene and the flanking homologous sequences
Name | Sequence (5′–3′) | |
|---|---|---|
1 | katA up600 fw | GCGGTGTTCCTGAAAAATAA |
2 | katA up600 rev | TGAATTTGTCATGTTATCACCTCTTGGAATTTATAAGAAC |
3 | katX2 fw | AGAGGTGATAACATGACAAATTCAAATCATAAAAATTTG |
4 | katX2 rev | TTGTTAATTAAATCAATTATTTCATGTTTCCTTGAAGGTAT |
5 | spec FW | GAAACATGAAATAATTGATTTAATTAACAACTATGGATATAAAATAG |
6 | spec rev | TGCATTTCTCCATTATTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAGTT |
7 | katA down600 fw | TAAAAAAATTATAATAATGGAGAAATGCAAAAACC |
8 | katA down600 rev | TTAAAAGGGAAAAGTTCTCATAGC |