Table 2 Efficiency of simultaneous two-loci mutagenesis (ompF 5′/ompF 3′ regions and fecA/lpp genes) using pCRISPR plasmids carrying REPEAT-gDNA₁-REPEAT-gDNA₂-REPEAT cassette

pCRISPR-ompF-5′G_ompF-3′I

ompF_5′G-120-∆30

∆30 ompF_5′

∆30 ompF_3′

∆30 ompF_5′/∆30 ompF_3′

10% (2/20)

Not tested

Not tested

pCRISPR-ompF-5′G_ompF-3′I

ompF_3′I-120-∆30

∆30 ompF_5′

∆30 ompF_3′

∆30 ompF_5′/∆30 ompF_3′

Not tested

0% (0/20)

Not tested

pCRISPR-ompF-5′G_ompF-3′I

ompF_5′G-120-∆30 + ompF_3′I-120-∆30

∆30 ompF_5′

∆30 ompF_3′

∆30 ompF_5′/∆30 ompF_3′

5% (1/20)

0% (0/20)

57% (12/20)

pCRISPR-lpp_B-fecA_B

lpp_B-120-∆30 + fecA_B-120-∆30

∆30 lpp

∆30 fecA

∆30 lpp/∆30 fecA

3% (1/29)

0% (0/29)

31% (9/29)