Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

Fig. 2

Selection of gDNAs for mutation of ompF, lpp and fecA genes. The grey bars are a schematic drawing of the genes lpp, fecA and ompF, and the black lines labelled with letters indicate the positions where the gRNAs transcribed from their corresponding gDNAs hybridize within each gene. gDNAs were cloned into pCRISPR, generating the plasmids reported in the Additional file 1: Table S1. The tables report the transformation efficiencies (CFU/μg) of each pCRISPR-gDNA in BL21(DE3)∆ompA(pCasRed)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us