Fig. 4

The a relative log10-transformed concentrations (% of total lipid content), and b log2 fold change (in comparison to the concentrations measured for the cultivations performed at 20 °C and 0% supplemented EtOH) of the relative concentrations (% of total lipid content), of the 60 lipid species determined by UPLC/MS lipidomics in the lipids extracted from cells in late exponential phase during the growth assays. Values are means from three independent cultivations. a The heatmap was generated based on the z-scores (a blue color indicates a negative z-score and lower concentration, while a red color indicates a positive z-score and a higher concentration). b A blue color indicates a decrease in concentration, while a red color indicates an increase in concentration compared to the cultivation at 20 °C and 0% supplemented EtOH. The asterisks indicate whether the change was significant as determined by Student’s t test (unpaired, two-tailed and unequal variance) corrected with the Benjamini-Hochberg procedure (*p < 0.05; **p < 0.01; ***p < 0.001)