Fig. 7
From: Application of an E. coli signal sequence as a versatile inclusion body tag
Expression and inclusion body formation of the toxin SymE in the absence and presence of fused ssTorA. a Growth curves for E. coli TOP10F’ cells expressing SymE or ssTorA/SymE as indicated. Cells were grown to an OD660 of 0.3 when expression of SymE or ssTorA/SymE was induced by addition of anhydrotetracycline. Error bars indicate standard deviation; n = 3. b Inclusion body formation of SymE and ssTorA/SymE in the cultures described under a analyzed and displayed as described in the legend to Fig. 4