Fig. 6
From: Application of an E. coli signal sequence as a versatile inclusion body tag
Expression of hEGF in the absence and presence of fused ssTorA. E. coli HDB37 cells were grown to early log-phase when expression of either hEGF or ssTorA/hEGF was induced by addition of anhydrotetracycline (ahtc). Samples were withdrawn from the cultures at the time point of induction (−ahtc) and 2 h after induction (+ahtc). Cells were analyzed by SDS-PAGE and Coomassie staining. Expression products hEGF and ssTorA/hEGF are indicated (*). Molecular mass (kDa) markers are indicated at the left side of the panels