Fig. 5
From: Application of an E. coli signal sequence as a versatile inclusion body tag
Microscopy analysis of IB formation. a E. coli TOP10F’ cells carrying an empty vector (EV) and cells expressing indicated proteins were analyzed by phase-contrast microscopy. Examples of IBs are indicated with a white arrow; examples of IBs seemingly formed at the new-pole of interconnected cells are indicated with a black arrow; examples of clustered IBs are indicated with a black arrowhead. Scale bar 2 μm. b Electron microscopy analysis of IBs isolated from TOP10F’ cells overexpressing indicated proteins. Representative IBs of average size are shown. Scale bar 500 nm