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Table 2 Specific activities of XR (xylose reductase), XDH (xylitol dehydrogenase), XK (xylulokinase), ADH (alcohol dehydrogenase), PDC (pyruvate decarboxylase), FBP (fructose-1,6-bisphosphatase), and TKL (transketolase) in the cells of analyzed O. polymorpha strains from third day of xylose alcoholic fermentation at 45 °C

From: Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha

Strain

Activity U/mg of protein

XR

XDH

XK

PDC

ADH

TKL

FBP

WT

0.012 ± 0.001

0.011 ± 0.001

–

0.165 ± 0.008

0.103 ± 0.005

0.005 ± 0.002

0.012 ± 0.001

cat8Δ

0.014 ± 0.002

0.006 ± 0.001

–

0.183 ± 0.012

0.119 ± 0.001

0.008 ± 0.001

0.014 ± 0.003

BEP

0.023 ± 0.003

0.335 ± 0.004

0.494 ± 0.031

0.323 ± 0.018

0.119 ± 0.020

0.012 ± 0.004

0.011 ± 0.001

BEP cat8Δ

0.028 ± 0.002

0.255 ± 0.018

0.629 ± 0.038

0.346 ± 0.006

0.189 ± 0.015

0.019 ± 0.003

0.015 ± 0.002

  1. – Not determined