Fig. 2

Effect of glucose or ammonium limitation on the metabolism of C. salexigens. Cells were grown at 2.5 M NaCl in M63 minimal medium with different ammonium to glucose ratios. [2-¹³C]-glucose was used as carbon source. The compatible solutes accumulated in the cells were extracted and the incorporation of the label at specific positions of the molecules was monitored using ¹³C-NMR and used to calculate metabolic flux ratios as described previously [6]. a Relative content of compatible solutes ectoine (white bars), hydroxyectoine (grey bars) and glutamate (black bars) in late exponential phase cells. b Selected metabolic flux ratios relevant for the functioning of the pyruvate node: pyruvate carboxylase (Pc) and pyruvate dehydrogenase (Pdh), citrate synthase (Cs) and ectoine synthase (EctA)