Fig. 6

Determination of reactive oxygen species (ROS) in Synechocystis sp. PCC 6803 acetate transporter expression strain (AT; red bars) in comparison to the control strain (CS; blue bars) grown for 8 days in MC1000 under continuous low light (20 μmol photons m⁻² s⁻¹). a Overall cellular ROS content measured immediately (0 timepoint) and after 45 min extension in the standard low light growth conditions using the membrane permeable fluorescence indicator CM-H₂DCFDA. The mean and standard deviation represent four biological replicates. b Production of singlet oxygen in the presence of 5 mM His upon illumination of the cells at 3000 μmol photons m⁻² s⁻¹. The oxygen uptake rate was calculated as µmol O₂ produced h⁻¹ mL⁻¹ (cell culture) at OD₇₅₀ = 1.5. The mean and standard deviation represent three biological replicates