Fig. 7From: Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strainsMaximal extracellular D1.3 scFv concentrations for recombinant B. subtilis 168, DB431 and WB800N. B. subtilis strains were transformed with plasmid pRBBm117 (P opt. xylA -sp lipA -d1.3scFv-his 6 ). Cultivation took place in microtiter plates (1250 μL culture volume), baffled shake flasks (150 mL culture volume) and stirred tank bioreactors (2 L culture volume) under aerobic conditions at 37 °C. All cultivation systems were inoculated with cell concentrations (cell dry weight) of 0.0337 g L−1 in minimal medium containing 10 mg L−1 of tetracycline. Recombinant D1.3 scFv secretion was induced by the addition of 5 g L−1 of xylose at cell concentrations of around 0.10 g L−1. Extracellular concentration of D1.3 scFv [mg L−1] for B. subtilis 168, DB431 and WB800N was analyzed using ELISA. Further analyses are shown in Additional file 1: Figures S3–S6Back to article page