Table 2 The editing efficiency and colony number at poxB locus with different length of homologous arms
From: Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
Homologous arm length | Proximate colony number | Editing efficiency |
|---|---|---|
297 bp plus 298 bp | 500 | 100 ± 0% |
101 bp plus 101 bp | 150 | 69.3 ± 1.17% |
51 bp plus 51 bp | 50 | 0 |