Table 1 The plasmids used for this work
From: Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
Plasmids | Relative characteristics | Resources |
|---|---|---|
pCas9 | Cas9 | [10] |
pKd46 | Exo, bet, gam, rep and arabinose operon | [3] |
pAra_Cas9_Δpoxb300 | Arabinose operon, Cas9, gRNA with N20 and with homologous arms of about 300 bp for poxb | This work |
pRed_Cas9_ΔpoxB300 | Derived from pKD46, exo, bet, gam, arabinose operon, Cas9, gRNA with N20 and with homologous arms of about 300 bp for poxb deletion | This work |
pRed_Cas9_Δpoxb::rfp300 | Derived from pRed_Cas9_ΔpoxB300, and with homologous arms of about 300 bp with rfp replace poxb | This work |
pRed_Cas9_Δpoxb100 | Derived from pRed_Cas9_ΔpoxB300, and with homologous arms of about 100 bp for poxb deletion | This work |
pRed_Cas9_Δpoxb50 | Derived from pRed_Cas9_ΔpoxB300, and with homologous arms of about 50 bp for poxb deletion | This work |
pRed_Cas9_recA_Δpoxb300 | Derived from pRed_Cas9_ΔpoxB300, recA | This work |
pRed_Cas9_recA_Δpoxb41 | Derived from Red_Cas9_recA_Δpoxb300, and with homologous arms of about 41 bp for poxb deletion | This work |
pRed_Cas9_recA_Δpoxb::rfp41 | Derived from Red_Cas9_recA_Δpoxb300, and with homologous arms of about 41 bp for rfp replace poxb | This work |
pRed_Cas9_recA_ΔlacZ41 | Derived from Red_Cas9_recA_Δpoxb300, and with homologous arms of about 41 bp for lacZ deletion | This work |