Fig. 2From: Elimination of N-glycosylation by site mutation further prolongs the half-life of IFN-α/Fc fusion proteins expressed in Pichia pastoris Expression of the IFN-α/Fc-MD fusion protein. The transformed colonies of IFN-α/Fc-MD were screened by dot blot (a); and further confirmed by Western blot (b). a1–c8: the colony number. c The time period for the expression of IFN-α/Fc-MD during induction in fermentation was analyzed by Western blot under reduced and non-reduced conditionsBack to article page