Fig. 4From: Siropins, novel serine protease inhibitors from gut microbiota acting on human proteases involved in inflammatory bowel diseasesDetermination of the RCL cleavage sites of the Siropin 1 and 2 using mass spectrometry analysis. a The stained protein bands labelled with frames were excised and subjected to mass spectrometry analysis. Siropin 1: M protein marker; 1 Siropin 1; 2 HNE; 3 Siropin 1 + HNE; 4 PR3; 5 Siropin 1 + PR3. Siropin 2: M protein marker; 1 Siropin 2; 2 HNE; 3 Siropin 2 + HNE; 4 PR3; 5 Siropin 2 + PR3. b Diagram of the reactive center loops showing the cleavage sites for each Siropin identified with HNE and PR3Back to article page