Fig. 5
Production of ATF-SAPs in bioreactor. Pichia pastoris growth curve (a), glycerol (b) and methanol (c) concentration were monitored before and after the induction of ATF-SAP WT (blue line) or ATF-SAP KQ (red line) expression. The light blue arrows indicate the pulses of methanol. Error bars represent standard deviations from the mean of triplicate samples. The chimeric fusion proteins secreted into the supernatants at the indicated time points were analyzed by western blotting using an anti-saporin antibody as probe (d). Bands were quantified and expressed as fold increase (e)