Table 2 Plasmids and S. cerevisiae strains used in this study
| Â | Description | Source |
|---|---|---|
Plasmid | ||
pY26TEF-GPD (pY26) | URA3/2μ yeast shuttle vector containing GPD1 promoter | [37] |
pY26-RHO1 | RHO1 expressed from GPD1 promoter in pY26TEF-GPD | This study |
pY26-FKS2 | FKS2 expressed from GPD1 promoter in pY26TEF-GPD | This study |
pRS424TEF | TRP1/2μ yeast shuttle vector containing TEF1 promoter | [38] |
pRS424TEF-LYZ | hLYZ expressed from TEF1 promoter in pRS424TEF | This study |
pRS424TEF-LYZ(R59S) | hLYZ(R59S) expressed from TEF1 promoter in pRS424TEF | This study |
Strains | ||
W303A-pY26 | As in W303-1A and blank pY26TEF-GPD | This study |
W303A-RHO1 | As in W303-1A and pY26-RHO1 | This study |
W303A-FKS2 | As in W303-1A and pY26-FKS2 | This study |
Δalg3Δoch1-RHO1 | As in Δalg3Δoch1 and pY26-RHO1 | This study |
Δalg3Δoch1-FKS2 | As in Δalg3Δoch1 and pY26-FKS2 | This study |
Δalg3Δoch1-pY26 | As in Δalg3Δoch1 and blank pY26TEF-GPD | This study |
Δalg3Δoch1-LYZ | As in Δalg3Δoch1 and pRS424TEF-LYZ | This study |
W303A-pY26-LYZ(R59S) | As in W303A with blank pY26TEF-GPD and pRS424TEF-LYZ (R59S) | This study |
Δalg3Δoch1-LYZ(R59S) | As in Δalg3Δoch1 and pRS424TEF-LYZ (R59S) | This study |
Δalg3Δoch1-pY26-LYZ (R59S) | As in Δalg3Δoch1 with pRS424TEF-LYZ (R59S) and blank pY26TEF-GPD | This study |
Δalg3Δoch1-RHO1-LYZ (R59S) | As in Δalg3Δoch1 with pRS424TEF-LYZ (R59S) and pY26-RHO1 | This study |