Fig. 6From: Functional inclusion bodies produced in the yeast Pichia pastoris In vitro internalization of VP1GFP IBs produced in E. coli (MC4100 strain) and P. pastoris (HY and LY clones). a Penetration into HeLa cells was measured by flow cytometry counting the percentage of fluorescent cells. b Mean fluorescence intensity was measured and the relative amount of VP1GFP into HeLa cells was estimated using the specific fluorescence (FU/µg) of VP1GFP IBs. Row flow cytometry data are shown in the Additional file 3 Back to article page