Fig. 3
PCR-based analysis of CYP61 mutants. a The diagram shows the amplification target and each set of primers. b The gel shows PCR reactions performed with different sets of primers and genomic DNA from the parental strain (WT), ΔCYP61(+, −) strain and ΔCYP61(−, −) strain. Lanes A, B, C, D and E represent the fragment between primers #1 and #3 (1.5 kbp), #2 and #4 (2.0 kbp), #1 and #5 (1.8 kbp), #2 and #6 (1.6 kbp), and #1 and #2 (7.5 kbp for CYP61 gene and 4.5 kbp for G418R gene), respectively. The estimated sizes of the PCR fragments were represented in parenthesis. Lane M represent DNA marker and numbers on the right side show molecular weights of DNA marker