Table 1 Strains and plasmids used and constructed in this study
From: Engineering Corynebacterium glutamicum for violacein hyper production
Strains/plasmids | Relevant characteristics | Sources |
|---|---|---|
Strains | ||
E. coli DH5α | F− endA1thi-1 recA1 relA1 gyrA96deoRΦ80dlacΔ(lacZ) M15 Δ(lacZYA-argF)U169hsdR17(r −K , m +K ) λ– supE44 phoA | Invitrogen |
ATCC 13032 | C. glutamicum wild-type | ATCC |
ATCC 21850 | 4-MTr 5-MTr 6-FTr 4-Apr 4-FPr TyrHxr Phe− Tyr−, tryptophan hyperproducer | ATCC |
Plasmids | ||
pEC-XK99E | C. glutamicum/E. coli shuttle expression vector, Ptrc, lacIq, Kanr | Add gene |
pEC-vioABCDE | derived from pEC-XK99E, constitutive expression of C. violaceum vio operon | This study |
pEC-J-vio-1 | pEC-XK99E derivative containing vio operon from J. lividum, expressed under control of inducible promoter Ptrc | This study |
pEC-J-vio-2 | pEC-XK99E derivative containing synthetic J. lividum vio operon with each gene containing complete C. glutamicum RBS sequence | This study |
pEC-C-vio-1 | pEC-XK99E derivative containing synthetic C. violaceum vio operon with each gene containing complete C. glutamicum RBS sequence | This study |
pEC-C-vio-2 | pEC-XK99E derivative containing synthetic C. violaceum vio operon with each gene containing complete C. glutamicum RBS sequence, which gene order changed to vioB, vioA, vioE, vioC, vioD | This study |