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Fig. 5 | Microbial Cell Factories

Fig. 5

From: In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011

Fig. 5

Optimized biotransformation of racemic (R,S)-PED to its (S)-isomer through a two-stage control strategy. The temperature and pH were set at 30 °C and 5.0 in 8 h, and then converted at 35 °C and 4.5 from 9 to 13 h. The recombinant C. parapsilosis/pCP-RCR cells catalyzed racemic (R,S)-PED to its (S)-isomer with a high optical purity (98.8 %) and a yield of 48.4 % within 13 h without addition of cofactors

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Microbial Cell Factories

ISSN: 1475-2859

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