Fig. 4From: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973The cas9 gene was present in early patches, but absent once curing had occurred. Primers were designed to sit on the pCRISPomyces-2 backbone, outside cas9, yielding a 4.252 kb product if the plasmid was presentBack to article page