Fig. 2From: The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli Overview of cultivation systems with integrated substrate supply. a Multilayer system by [17, 21]. A medium-agar was covered by a broth in which the bacteria were cultivated. b Feed Bead® System by Jeude et al. [19]. Silicone elastomer discs (feed beads) releasing substrate to the medium. c Principle of the integrated substrate delivery system with the EnBase technology [11]. Dissolved polysaccharides being part of the cultivation medium are degraded to glucose molecules by the addition of defined enzyme concentrationBack to article page