Fig. 2From: Increasing the production of (R)-3-hydroxybutyrate in recombinant Escherichia coli by improved cofactor supplyKinetic model of His-tagged acetoacetyl-CoA reductase synthesized by gene rx that was obtained from—the H. boliviensis. Symbols refer to NADH (squares) and NADPH (circles). The reaction rates at different concentrations of NADH and NADPH, respectively, were curve fitted to the Michaelis–Menten equation to obtain the values of Vmax and KM for each cofactorBack to article page