Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Table 1 List of strains with the highest specific antibody product yields

Strain number	Added elements	Strain	Specific product yield (µg ml⁻¹OD600⁻¹)	Relative frequency^a	P value^b	Fold-change relative to wild-type^c
154	CPR5-P _GPD	Δopi1	0.2027	0.2	0.000002	10.22
157	CPR5-P _KAR2	Δopi1	0.1992	0.15	0.000159	10.15
155	CPR5-P _GAL1	Δopi1	0.1579	0.1	0.000560	7.99
153	CPR5-P _TEF	Δopi1	0.1525	0.075	0.000036	7.66
156	CPR5-P _PDI1	Δopi1	0.1424	0.05	0.007624	7.15
170	ERO1-P _GAL1	Δopi1	0.1226	0.05	0.020502	6.13
212	SIL1-P _GAL1	Δopi1	0.1223	0.05	0.021128	5.53
162	PDI1-P _TEF	Δopi1	0.1208	0.075	0.279237	6.09
206	LHS1-P _GPD	Δopi1	0.1163	0.05	0.299870	5.88
402	LHS1-P _KAR2 + SIL1-P _KAR2	Δopi1	0.1139	0	0.011316	5.73
83	None	Δopi1	0.0886	0.075		4.47
73	None	wt	0.0199	0		1

The data represent the mean value of the experiments conducted with 0.5 and 2 % galactose induction at 30 °C
^aRelative frequency in the 95th percentile of Δopi1-strains
^bCalculated with the nonparametric equivalent of t test, Wilcoxon signed rank test using the Δopi1-strain without added elements as the reference
^cAverages of the fold-changes relative to wild-type

ISSN: 1475-2859