Fig. 4

Signal peptide cleavage sites. N-terminal sequence analysis of purified secreted proteins was performed by Edman degradation. The sequences of the native Bacillus signal peptides and the E. coli OmpA signal peptide are underlined. Arrows indicate the cleavage sites as deduced from sequence analysis. The first five amino acids obtained by Edman degradation are colored and boxed. Amino acids in red indicate extra amino acids that were introduced during genetic engineering of the fusions between the Bacillus enzymes and the E. coli OmpA signal peptide